Attention US and Canada Customers

Attention: Due to our annual physical inventory, orders received after 4PM CST on Tuesday, March 11th will be shipped according to the following schedule:
US Orders: Shipped on Saturday, March 15th with an expected arrival on Monday, March 17th.
Canada Orders: Shipped on Monday, March 17th with an expected arrival on Tuesday, March 18th.
We apologize for any inconvenience this may cause.
For any questions, please contact Customer Service at 1-800-356-9526.

HaloTag® Technology: Aqueous-Soluble Ligands Technical Manual

Instructions for Use of Product(s)
HT1010, HT1020, HT1030, HT1040, HT1050, HT1060, HT1070, HT1080, HT1090, HT1100, HT1110, HT1120, HT1130

Literature # TM753

The HaloTag® Technology platform addresses the need for flexibility in functional protein analysis. This modular technology is based on the formation of a covalent bond between a fusion tag, HaloTag® reporter protein, and synthetic ligands, and enables complete characterization of protein function in cellular and biochemical environments. There are a variety of HaloTag® Ligands and kits available for fluorescent cell imaging and protein capture/purification, as well as reactive customizable Ligand Building Blocks. The ligands described are soluble in cell media or aqueous buffers.

The modular nature of HaloTag® technology and the rapid binding kinetics with its ligands can directly image both protein trafficking and turnover. The available ligands have both varied fluorescent spectra and also cell-permeant and impermeant properties to provide spatial and temporal control. For example, you can label only the surface pool of a membrane-bound protein with a cell-impermeant ligand and follow its biology, internalization or other fate. Or a protein can first be labeled with a ligand that has one dye color, and after a period of time labeled again with a ligand containing a dye of a different color. Such pulse-chase labeling allows direct observation of protein trafficking and/or turnover over time.

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Experienced User Protocols