pCI-neo Mammalian Expression Vector
Constitutively Express Cloned Insert for Transient or Stable Transfection
- Select for stably transfected cells with neomycin phosphotransferase gene
- Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
- Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication
Catalog Number:
Size
Catalog Number: E1841
The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.
This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.
References
- Brinster, R.L. et al. (1988) Proc. Natl. Acad. Sci. USA 85, 836–40.
- Choi, T. et al. (1991) Mol. Cell. Biol. 11, 3070–4.
- Palmiter, R.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 478–82.
- Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.
pCI-neo Mammalian Expression Vector GenBank® Accession Number U47120 and vector sequence text file.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pCI-neo Mammalian Expression Vector |
E184A | 1 × 20μg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
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