Live-Cell Protein Interactions

Protein:protein interactions (PPIs) are essential elements of cellular signal transduction networks. Although numerous approaches exist to monitor PPIs in vitro, methods for intracellular detection have been more limited.

The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in vivo using BRET (NanoBRET®) or complementation reporter-based (NanoBiT®) methods.

The NanoBRET® and NanoBiT® technologies featured here enable detection of protein interactions at endogenous expression levels in ways that have not been possible before. The reversible assay technologies allow you to study both induction and inhibition of protein interactions using either NanoBRET® or NanoBiT® methods in live-cell assay formats that allow for interaction kinetics to be monitored in real time. Both technologies are compatible with bench scale or high-throughput, multiwell plate methods.