ECL Western Blotting Substrate
Chemiluminescent Substrate to Detect Horseradish Peroxidase (HRP) Conjugates on Immunoblots
- Detect picogram levels of protein with minimal background
- Non-radioactive
- No optimization required; easily switch from other entry-level ECL substrates
Catalog Number:
Size
Catalog Number: W1001
Catalog Number: W1015
Highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) conjugates on immunoblots. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods.
No optimization required; easy to switch from other entry-level ECL substrates.
Applications- Western blotting
- Dot blotting
Western blot (immunoblot) for β-actin in cytoplasmic lysate from HEK293T cells. The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of β-actin (ng) as quantitated via ELISA. Primary antibody (monoclonal) used at 1:5,000, Anti-Mouse IgG, HRP Conjugate, secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with ECL Western Blotting Substrate (Cat.# W1015) and with 1-minute exposure.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Peroxide Solution |
W100A | 1 × 125ml |
|
Luminol Enhancer Solution |
W101A | 1 × 125ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Peroxide Solution |
W100B | 1 × 250ml |
|
Luminol Enhancer Solution |
W101B | 1 × 250ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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