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J. Biol. Chem. 278(34), 31895-31901. Down-regulation of hypoxia-inducible factor-2 in PC12 cells by nerve growth factor stimulation. 2003

Naranjo-Sua’rez, S., Carmen Castellanos, M., A´lvarez-Tejado, M., Vara,A., Landa´zuri, M.O. and del Peso, L.

Notes: In this paper, the authors describe use of ImProm-II™ Reverse Transcriptase to transcribe cDNAs for Quantitative RT-PCR.  Reverse transcription (RT) reactions were performed with 1μg total RNA from treated rat PC12 cells. Light Cycler reactions were setup with 1-3μl cDNA from the RT reactions. The researchers incubated cells with EGF and bFGF and analyzed HIF-2α mRNA levels. For these experiments, PC12 cells were incubated with 30ng/ml EGF or 50μM bFGF for 8 hours.  Erk1&2 phosphorylation was examined by Western blot using the Anti-ACTIVE® MAPK pAb. Western results were visualized by chemiluminesent detection and analyzed with a digital luminescent image analyzer. The Dual-Luciferase® Reporter Assay System was used to analyze PC12 cell-expressed luciferase from pEpoEm1-luc and pEpoE-luc promoter constructs that were normalized with the pRL-TK vector.  Cells were harvested 17-18 hours post transfection and treatment.  (2725)

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Biochem. J. 338, 637-642. Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation. 1999

Nissen, N. N. , Shankar, R. , Gamelli, R. L. , Singh, A. , DiPietro, L. A.

Notes: Basic FGF was glycosylated by non-enzymatic means and tested for HUVEC cell proliferation. The proliferation was measured with the procedure of the CellTiter 96® AQueous Non-Radioactive Assay using reagents prepared for MTS Powder (Promega) and phenazine methosulphate (PMS; Sigma). (0621)

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J. Cell Biol. 143, 849-859. Dystrophic muscle in mice chimeric for expression of alpha5 integrin 1998

Taverna, D., Disatnik, M.-H., Rayburn, H., Bronson, R.T., Yang, J., Rando, T.A., Hynes, R.O.

Notes: The basic Fibroblast Growth Factor was used to maintain primary cultures of limb muscles of neonatal chimeric mice. (0255)

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rhFGF, Basic

Biochemistry 37, 6857-6863. Inhibition of human angiogenin by DNA aptamers: nuclear colocalization of an angiogenin-inhibitor complex. 1998

Nobile, V. , Russo, N. , Hu, G. f. , Riordan, J. F.

Notes: The authors used SELEX to identify specific sequence aptamers that bind to angiogenin and inhibit both its ribonucleolytic and angiogenic activities. Various Promega products were used, including Taq DNA polymerase and TetraLink™ Tetrameric Avidin Resin, to isolate sequences amplified with biotin-tagged antisense primers. For assaying the effect of the aptamers on the activity of angiogenin, human umbilical vein endothelial cells were grown on fibronectin coated dishes in human endothelial serum-free medium supplemented with 20ng/ml basic FGF. Angiogenin was added to these cells with or without prior pre-mixing with aptamers. (0625)

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rhFGF, Basic

J. Biol. Chem. 273, 19560-19565. Involvement of microphthalmia in the inhibition of melanocyte lineage differentiation and of melanogenesis by agouti signal protein. 1998

Aberdam, E., Bertolotto, C., Sviderskaya, E.V., de Thillot, V., Hemesath, T.J., Fisher, D.E., Bennett, D.C., Ortonne, J.P. and Ballotti, R.

Notes: The authors used the basic FGF (bFGF or FGF-2) in the culture medium to maintain the murine melb-a immortal melanoblast cell line. (2052)

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rhFGF, Basic

J. Cell Biol. 143, 2033-2044. Molecular organization of sarcoglycan complex in mouse myotubes in culture 1998

Chan, Y.M., Bönnermann, C.G., Lidov, H.G.W., Kunkel, L.M.

Notes: The basic Fibroblast Growth Factor (rhFGF, Basic) was used to maintain primary mouse skeletal myoblasts in culture. (1356)

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rhFGF, Basic

J. Biol. Chem. 273, 22317-22325. Nerve growth factor regulation of m4 muscarinic receptor mRNA stability but not gene transcription requires mitogen-activated protein kinase activity. 1998

Lee, N.H., Malek, R.L.

Notes: The SP6 and T7 RNA Polymerases were used to produce RNA probes for Northern Analysis. The authors examined the half-life of the m4 muscarinic receptor in PC12 cells and transformed PC12 cells following treatment with either murine 2.5S NGF, human recombinant basic FGF or human recombinant EGF. PC12 cells were also treated with the three factors, nuclei isolated, and nuclear run-on transcription assay were performed. (0814)

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J. Biol. Chem. 273, 2336-2343. Protein kinase C-dependent tyrosine phosphorylation of p130cas in differentiating neuroblastoma cells. 1998

Fagerstrom, S. , Pahlman, S. , Nanberg, E.

Notes: Used Promega Basic Fibroblast Growth Factor (bFGF, FGF-2) with the neuroblastoma cell line SHSY5Y. (1184)

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rhFGF, Basic

Proc. Natl. Acad. Sci. USA 94, 2204-2209. A putative angiogenin receptor in angiogenin-responsive human endothelial cells. 1997

Hu, G.F., Riordan, J.F., Vallee, B.L.

Notes: The authors used rhFGF, Basic, and anti-acidic FGF antibody (antibody has been discontinued) to analyze the effect of these factors on the activity of angiogenin on endothelial cells. They also labeled surface molecules of endothelial cells with biotin following treatment with angiogenin to aid in purification of angiogenin receptor. Then they took biotin-labeled molecules and purified using SoftLink™ Soft Release Avidin Resin, detected proteins on Western blot using Streptavidin Alkaline Phosphatase, and grew human umbilical arterial endothelial cells on Fibronectin-coated dishes. (1018)

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J. Cell Biol. 139, 507-515. Actinin-associated LIM protein: identification of a domain interaction between PDZ and spectrin-like repeat motifs 1997

Xia, H. , Winokur, S. T. , Kuo, W. L. , Altherr, M. R. , Bredt, D. S.

Notes: Promega's Basic Fibroblast Growth Factor (rhFGF, basic) was used as a culture supplement for the C2 myogenic cell line. (0162)

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rhFGF, Basic

J. Mol. Neurosci. 8, 29-44. On the role of the low-affinity neurotrophin receptor p75LNTR in nerve growth factor induction of differentiation and AP1 binding activity in PC12 cells. 1997

Kontny, E., Ciruela, F., Svenningsson, P., Ibáñez, C.F. and Fredholm, B.B.

Notes: The inhibitor was used to protect RNA during RT-PCR. 125I-NGF was produced and used to crosslink to cell surface proteins on PC12 cells. The NGF was also used to assay for neurite outgrowth of PC12 and two clonal variants. Cells were treated with NGF and nuclear extracts made and used to assay for AP1 binding activity. Tyrosine phosphorylation of PI kinase in response to NGF was assayed. Also, the concentration-dependent effect of NGF on the accumulation of 3H-inositol phosphate was examined in the PC12 cells. The bFGF was used to assay the induction of cell differentiation in the PC12 cell and a clonal variant. (1632)

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