Promega Corporation

Automated Plasmid Purification Using MagneSil™ Paramagnetic Particles

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Abstract

The Wizard® MagneSil™ Plasmid Purification System provides a reliable high-throughput method for plasmid DNA purification in a 96-well format. Here we present protocols for processing multiple plates of DNA samples with no manual intervention for multiple robotic platforms. Plasmid DNA yields of 6–9µg are routinely obtained. Purified DNA is ready-to-use in automated fluorescent sequencing.

Promega Notes 79, 22–24.

Gary Shiels, Doug White and Don Smith
Promega Corporation
Publication Date: 2001

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