The Steady-Glo™ Luciferase Assay System provides single-step quantitation of firefly luciferase expression in mammalian cell culture with extended-lifetime luminescence. The reagent is compatible with a broad range of culture media and is optimized for 96- and 384-well plates. Compared with conventional reagents having extended-lifetime luminescence, its unique formulation is more tolerant of mixing conditions, sample evaporation and pipetting errors. Thus, the Steady-Glo™ Reagent more readily supports good assay precision, particularly when used with automated sample handling instruments. These characteristics make the Steady-Glo™ Reagent particularly well suited for high-throughput batch analysis.
Promega Notes 70, 7.
Erika Hawkins, Martha Jennens-Clough, and Keith V. Wood