Riboprobe® Systems

The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.

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The Riboprobe® Systems are designed for in vitro preparation of high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts. These systems contain all components necessary for in vitro transcription from a DNA template (excluding the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for template removal following transcription.

Features - Benefits

• Specific: SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template.
• Choice of Enzyme: Systems available with SP6 RNA Polymerase, T7 RNA Polymerase or T3 RNA Polymerase.
• Convenient: Includes positive control template for use with SP6, T7 or T3 RNA Polymerase, DNase I for removal of DNA template and Recombinant RNasin® Ribonuclease Inhibitor.

Applications

• Generation of high-specific-activity RNA probes.
• Generation of microgram quantities of RNA transcripts.
• Generation of RNA processing substrates.
• Generation of antisense RNA.

For more information, see the Protocols & Applications Guide.

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