Bright-Glo™ Luciferase Assay System

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. These attributes also benefit scientists who are using fewer samples but still require high sensitivity and ease of use.

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High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. These attributes also benefit scientists who are using fewer samples but still require high sensitivity and ease of use.

Features - Benefits

• No Sample Preprocessing: No need to remove culture medium or wash cells prior to adding assay reagent. Grow cells and assay them directly within the same multiwell plate.
• Increased Sensitivity: Up to tenfold more light intensity than other homogeneous luciferase assay reagents.
• Improved Assay Precision and Reproducibility: Less sensitive to mixing conditions, sample evaporation and pipetting errors.
• Convenience: Simply mix buffer with lyophilized substrate and add to cells in culture medium; no need to thaw or measure before use.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

For more information, see the Protocols & Applications Guide.

Notes

Please contact Promega for information on bulk purchases.

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