The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express® Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection. All ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative.
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For many applications, AP conjugates are superior to HRP conjugates because they:
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ProtoBlot® II AP System with Stabilized Substrate, Human
Anti-Human IgG (H&L) AP Conjugate
Western Blue® Stabilized Substrate for Alkaline Phosphatase
ProtoBlot® II AP System with Stabilized Substrate, Mouse
Anti-Mouse IgG (H&L) AP Conjugate
ProtoBlot® II AP System with Stabilized Substrate, Rabbit
Anti-Rabbit IgG (Fc) AP Conjugate
Store antibody conjugates at 4°C (undiluted). Store Western Blue® Substrate at room temperature.
For product intended use please see Patents & Disclaimers tab.