Eliminate missed cleavages. Generate an increased number of peptides and protein identifications.
Use plasmid or PCR DNA as templates to express full-length, soluble, functional protein in 1-4 hours with the TnT® cell-free systems. No requirement to reclone; use standard vectors containing either a T7, T3 or SP6 promoter.
Express up to 500µg/ml protein directly from circular plasmid DNA using a cell-free E.coli-based system. No requirement for special equipment. Kits also available for linear or PCR DNA templates.
Convenient mammalian systems and competent cells designed for easy, controlled protein expression.
Utilize PNGase F and Endo H to characterize protein glycosylation. Typical applications include monitoring protein trafficking or determining location and structure of glycans by mass spectrometry.
Proteases available for optimal digestion applications include: Trypsin, Chymotrypsin, rLys-C, Lys-C, Asp-N, Glu-C and others. ProteaseMAX™ Surfactant is available for improved in-gel digestion and protein solubilization.
Mass spectrometry services (in collaboration with MS Bioworks) options include: protein identification, protein molecular weight determination, post-translation modification analysis, glycosylation characterization and protein quantitation.
Cell imaging, in vivo imaging and protein localization systems. Fluorescent and colorimetric labeling systems for immunoblotting.