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GoTaq® Probe 1-Step RT-qPCR SystemPCR Satisfaction Guaranteed

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions. The GoTaq® Probe qPCR Master Mix included ...

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GoTaq® Probe 1-Step RT-qPCR System

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GoTaq® Probe 1-Step RT-qPCR System

 Components

  • GoTaq® Probe Master Mix with dUTP

    A612A2 x 1ml
  • CXR Reference Dye

    C541B1 x 200μl
  • GoScript™ RT Mix for 1-Step RT-qPCR

    M700A1 x 225μl
  • Nuclease-Free Water

    P119A2 x 1,250μl
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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200 reactions
- A6120 SEK 4.944,00 Add to cart


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Storage Conditions

Store all components between –30°C and –10°C. Protect components from light at all times. For best results, mix thawed solutions gently to minimize aeration and foaming, and keep on ice. For short-term storage and frequent use, the GoTaq® qPCR Master Mix, 2X, may be kept at 2–8°C for up to 3 months if protected from light.

For product intended use please see Patents & Disclaimers tab.

Detection of GAPDH from human control RNA (100ng–0.1pg) using GoTaq® Probe 1-Step RT-qPCR System.Figure 1. Detection of GAPDH from human control RNA (100ng–0.1pg) using GoTaq® Probe 1-Step RT-qPCR System.

Panel A. Cq values for 6 × tenfold dilution series of human control RNA detecting GAPDH in a 20μl 1-step RT-qPCR reaction comparing GoTaq® Probe 1-Step RT-qPCR System to a 1-Step RT-qPCR master mix from Vendor L. GoTaq® Probe 1-Step RT-qPCR System gave earlier Cq values with the pg of RNA samples showing greater sensitivity. Panel B. Amplification curve for the 6 × tenfold dilution series of human control RNA detecting GAPDH with the GoTaq® Probe 1-Step RT-qPCR System. The qPCR reaction efficiency was calculated to be 102.2% with an r2 of 0.998.

Panel A. Cq values for 6 × tenfold dilution series of human control RNA detecting GAPDH in a 20μl 1-step RT-qPCR reaction comparing GoTaq® Probe 1-Step RT-qPCR System to a 1-Step RT-qPCR master mix from Vendor L. GoTaq® Probe 1-Step RT-qPCR System gave earlier Cq values with the pg of RNA samples showing greater sensitivity. Panel B. Amplification curve for the 6 × tenfold dilution series of human control RNA detecting GAPDH with the GoTaq® Probe 1-Step RT-qPCR System. The qPCR reaction efficiency was calculated to be 102.2% with an r2 of 0.998.

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Use Restrictions

A6120 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

A6120 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

A6120 NOTICE TO PURCHASER: DISCLAIMER OF LICENSE
No license is conveyed with the purchase of this product under any of US Pat. Nos. 5,210,015, 5,487,972, 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787 and 6,258,569 and corresponding patents outside the United States or any other patents or patent applications relating to the 5´ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 USA.

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