Systems that allow easy setup of sensitive and efficient long PCR amplifications are powerful tools, opening up new applications for PCR including physical mapping and direct cloning from genomes.
Learn about the new GoTaq® Long PCR Master Mix
GoTaq® Long PCR Master Mix
Amplify fragments of up to 40kb from lambda or 30kb from human genomic DNA. This specially formulated master mix contains a blend of the hot start polymerase and proofreading enzyme, allows simple reaction setup at room temperature and enhances yield, sensitivity and specificity.
This chapter of the Protocols and Applications Guide provides protocols and background information about PCR and RT-PCR.
A protocol for amplification of long PCR targets with a ready-to-use master mix containing all you need for long PCR. Simply add template and primers!