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Recombinant RNasin® Ribonuclease Inhibitor

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RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www....

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Recombinant RNasin® Ribonuclease Inhibitor

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Recombinant RNasin® Ribonuclease Inhibitor

 Components

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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 2,500u
 20–40u/μl N2511 SEK 1.322,00 Add to cart

Recombinant RNasin® Ribonuclease Inhibitor

Close Window

Recombinant RNasin® Ribonuclease Inhibitor

 Components

  • Recombinant RNasin® Ribonuclease Inhibitor

    N251B1 x 10,000u
Close Window

  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Login to request to add this product to your Helix »


 10,000u
 20–40u/μl N2515 SEK 3.169,00 Add to cart


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QC Tests

SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source

Recombinant strain of E. coli (Cat.# N2511, N2515).

Storage Buffer

20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.

Figures

Gel analysis of RNA integrity after incubation.Figure 1. Gel analysis of RNA integrity after incubation. Nine samples of water and buffer stocks from an RNase-free zone of an academic lab were tested for RNases. Samples were divided into two aliquots and incubated overnight at 37°C without (Panel A) or with (Panel B) Recombinant RNasin® Ribonuclease Inhibitor. Seven of the nine stocks contained RNases.
11089TA_1080pxFigure 2. Gel images of RNA samples created by the Agilent Bioanalyzer system.

We analyzed samples with varying concentrations of Recombinant RNasin® Ribonuclease Inhibitor before and after six weeks of storage with repeated freeze-thaw cycles. Panel A. Represents samples before storage. Panel B. Represents samples after the six-week period. The gel models demonstrate that the samples show little RNA degradation.

We analyzed samples with varying concentrations of Recombinant RNasin® Ribonuclease Inhibitor before and after six weeks of storage with repeated freeze-thaw cycles. Panel A. Represents samples before storage. Panel B. Represents samples after the six-week period. The gel models demonstrate that the samples show little RNA degradation.

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Comparison of RNasin® Ribonuclease Inhibitor and RNaseOUT™ inhibition of RNase A during in vitro transcription.Figure 3. Comparison of RNasin® Ribonuclease Inhibitor and RNaseOUT™ inhibition of RNase A during in vitro transcription.

pGEM® Express Control Template was transcribed using the RiboProbe® System—T7 following the instructions in Technical Manual #TM016. All reactions were performed in duplicate. Expected sizes in bases are displayed on the right of the gel image, and marker sizes in kb are listed on the left. The graph was generated by quantitating the bands on the gel and calculating a percent RNA compared to the positive control (polymerase + template). Error bars represent the two separate reactions. The black bars are quantitation of the top bands (2,346 bases) and the grey bars are quantitation of the bottom bands (1,065 bases).

Use Restrictions

N2511, N2515 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Patents - Disclaimers

U.S. Pat. No. 5,552,302.

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