Promega Corporation

Untagged Flexi® Mammalian Expression Vectors

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence.

The vectors below are designed specifically for high-level expression of proteins in mammalian cells from the CMV promoter with or without a selectable marker. The pFN9A Vector provides Renilla luciferase, which may be used as a transfection control. The pFN9A Vector was designed ...

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  • Prices valid for customers of Promega Biotech AB only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

pF4A CMV Flexi® Vector

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pF4A CMV Flexi® Vector

 Components

  • pF4A CMV Flexi® Vector

    C848A1 x 20μg
20μg
- C8481 SEK 3.129,00 Add to cart

pF4K CMV Flexi® Vector

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pF4K CMV Flexi® Vector

 Components

  • pF4K CMV Flexi® Vector

    C849A1 x 20μg
20μg
- C8491 SEK 3.129,00 Add to cart

pF5A CMV-neo Flexi® Vector

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pF5A CMV-neo Flexi® Vector

 Components

  • pF5A CMV-neo Flexi® Vector

    C940A1 x 20μg
20μg
- C9401 SEK 3.129,00 Add to cart

pF5K CMV-neo Flexi® Vector

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pF5K CMV-neo Flexi® Vector

 Components

  • pF5K CMV-neo Flexi® Vector

    C941A1 x 20μg
20μg
- C9411 SEK 3.129,00 Add to cart

pF9A CMV hRluc-neo Flexi® Vector

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pF9A CMV hRluc-neo Flexi® Vector

 Components

  • pF9A CMV hRluc-neo Flexi® Vector

    C936A1 x 20μg
20μg
- C9361 SEK 3.129,00 Add to cart


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GenBank®/EMBL Accession Number

pF4A CMV Flexi® Vector Accession Number AY753580; pF4A CMV Flexi® Vector sequence text file.
pF4K CMV Flexi® Vector Accession Number AY753581; pF4K CMV Flexi® Vector sequence text file.
pF5A CMV-neo Flexi® Vector Accession Number DQ487155; pF5A CMV-neo Flexi® Vector sequence text file.
pF5K CMV-neo Flexi® Vector Accession Number DQ487156; pF5K CMV-neo Flexi® Vector sequence text file.
pF9A CMV hRluc-neo Flexi® Vector Accession Number DQ871024; pF9A CMV hRluc-neo Flexi® Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

pF4A CMV Flexi Vector.Figure 1. pF4A CMV Flexi® Vector.
pF4K CMV Flexi Vector.Figure 2. pF4K CMV Flexi® Vector.
pF5A CMV-neo Flexi Vector.Figure 3. pF5A CMV-neo Flexi® Vector.
pF5K CMV-neo Flexi Vector.Figure 4. pF5K CMV-neo Flexi® Vector.
pF9A CMV hRluc-neo Flexi Vector.Figure 5. pF9A CMV hRluc-neo Flexi® Vector.

Use Restrictions

C8481, C8491, C9401, C9411, C9361 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

C9361 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

C8481, C8491, C9401, C9411, C9361 European Pat. No. 1685247 and other patents pending.

C8481, C8491, C9401, C9411, C9361 Patent Pending.

C9361 U.S. Pat. No. 7,906,282 and European Pat. No. 1341808.

C8481, C8491, C9401, C9411, C9361 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

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